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Production "h_biblio_ref" : Ito et al., Int J Oncol, 4: 47 (1994) [PMID: ] ..............................................^ no match with |[0-9]+|
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CABRI:DSMZ_MUTZ
Accession_number ACC 133
Cell_line_name 8305C
Brief_description confirmed as human with IEF of AST, MDH, NP established from the primary tumor of a 67-year-old woman with primary thyroid carcinoma (undifferentiated carcinoma, composed of spindle, polygonal and giant cells)
Description human thyroid carcinoma established from the primary tumor of a 67-year-old woman with primary thyroid carcinoma (undifferentiated carcinoma, composed of spindle, polygonal and giant cells) confirmed as human with IEF of AST, MDH, NP
Depositor Dr. M. Akiyama, Radiation Effects Research Foundation, Hiroshima, Japan
Morphology adherent, large spindle-shaped cells growing as monolayer
Culture_conditions 80-90% RPMI 1640 + 10-20% FBS split confluent culture 1:5 once a week using trypsin/EDTA; seed out at about 1 x 106 cells/175 cm 2 at 37C with 5% CO2 about 5-6 x 106 cells/175 cm 2
Viruses ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, SMRV -
Properties cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM -, GFAP +, neurofilament -, thyroglobulin -, vimentin +
Release_conditions -
Hazard -
Storage frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule
Doubling_time about 54 hours
Mycoplasma negative in DAPI, microbiological culture, RNA hybridization assays
Fingerprint multiplex PCR of minisatellite markers revealed a unique DNA profile
Cytogenetics human flat-moded hypertriploid karyotype with 22% polyploidy - 74(67-76)<3n>XXX, -2, +9, -10, -12, -13, +14, +15, +15, +18, -19, +20, +4mar, 1-20 dm in most cells, der(6)t(6;7)(q22;q36q11.23), add(15)(p11)x2, del(17)(p12)x2 - subclonal rearrangements, e.g. del(2)(p16), del(2)(q22), del(3)(q22), i(4p), t(6p;7q), del(9)(p23)
Bibliographic_reference Ito et al., Int J Oncol, 4: 47 (1994) [PMID: ]
SRS Release 7.1.3    Copyright © 1997-2003 LION bioscience AG. All Rights Reserved. Terms of Use Feedback